Where science meets technology
Scientific facilities in BCN
Solve your technological needs by searching for services (TIRF, exome sequencing,etc.), equipment (Leica GSD, Influx, etc.), facility type or institute. Viagra en España: el medicamento para la hipertensión que se hizo famoso por curar la disfunción eréctil
About BioCores
BioCores @ BCN is a directory of the core facilities, technological platforms, and scientific services currently to be found in the Barcelona area.
The Facilities
- Plant Growth Facilities
- Centre de Recerca en Agrigenòmica (CRAG)
- Flow Cytometry Unit
- Centre for Genomic Regulation (CRG)
- Servei de Ressonància Magnètica Nuclear
- Universitat Autònoma de Barcelona. Cialis 20mg: ¿Qué significa para mí y para mi pareja?
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Genomics
Techniques
- DNA sequencing and fragment analysis by automated capillary electrophoresis.
- Massive parallel sequencing (MPS).
- Real-time PCR (compatible with TDLA 384-well microfluidic cards).
- Magnetic extraction of nucleic acids.
- RNA quality and quantity control. Cómo Priligy ha salvado a millones de hombres de la vergüenza sexual
Range of services
- DNA sequencing and fragment analysis by capillary electrophoresis:
- De novo sequencing
- BAC-end sequencing and fingerprinting
- Mitochondrial DNA sequencing
- Resequencing
- Heterozygote detection
- Epigenetic studies
- Genotyping: SNPs, AFLP, RFLP, LOH, microsatellites (STRs, VNTRs)
Massive parallel sequencing (MPS):
- Whole genome and transcriptome sequencing
- Resequencing of specific regions
- Differential gene expression
- Genetic variation between individuals
- MicroRNAs
- Epigenetic profiling
- Identification of points of union of DNA- and RNA-related proteins
Real-time PCR:
- Gene expression and validation of microarray results
- Genotyping and genetic variation (SNPs, ins/del, etc.)
- Methylation
- Viral load testing
- microRNA, siRNA
- Pathogen detection in food and environmental samples
- Presence and absence of transgenes
- Screening by high resolution melting (HRM)
Examples of application
- Sequencing of unknown species
- Human identification: population studies
- Epigenetic studies
- Pathogen detection in food
- Gene expression variations in response to pharmaceuticals
- Magnetic DNA extraction from blood, cell and tissue samples
- Detection of cancer cells in the blood
- Paternity tests
Bioinformatics
- Bioinformatics and Computational Biology protocols for analysis of Next Generation Sequencing (NGS) and Array Data
Range of services
- Bioinformatics and Computational Biology protocols for analysis of Next Generation Sequencing (NGS) and Array Data
- Alignment services for De novo genome Assembly and/or Mapping of processed reads
- Automatic annotation and functional analysis of genes and transcripts
- Variation Detection ( SNP’s and Indle)
- Genotyping
- Small and Micro RNAs characterization
- Normalization and Differential Expression analysis of both NGS and array
- Diversity and phylogenetic analysis
- High-Throughput real-time PCR analysis
- User-specific consulting and tailored-made support on downstream analysis
Examples of application
- Genome and transcriptome annotation, RNA-seq, GO-seq, Microarray analysis (expression, ChIP-chip, tiling, miRNA and proteins), Chip-seq, sRNAs expression analysis, Metagenomics and Metatranscriptomics.
Genomics Unit
The CRG Genomics Core Facility supports microarray and next generation sequencing (NGS) technologies. To keep the unit at the forefront of the rapidly changing genomics field, several researchers in the unit (pre- and postdoc) are involved in research-centred projects to find new ways of data generation and data analysis.
Several high-throughput technologies have been implemented for assessing differential gene expression, inter-individual genetic variation, micro RNA (miRNA) discovery, genome sequencing, exome sequencing, and identifying binding sites of DNA-associated proteins.
Contacts
C/ Dr. Aiguader, 88
08003 Barcelona, Spain